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June 2023 Living Environment Regents — Answer Key & Worked Solutions

Every question from the June 2023 Living Environment Regents exam, with the correct answer and a full step-by-step solution. Check your work, learn the method, then convert your raw score.

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These are real questions from the June 2023 Living Environment New York State Regents examination, published by NYSED. Worked solutions are written by RegentsLab.

Question 1 · 2 pts

Which two body systems provide humans with the raw materials necessary for their cells to release energy?

Worked solution
$\text{Cellular respiration requires glucose and oxygen}$
$\text{Digestive system breaks down food into glucose}$
$\text{Respiratory system supplies oxygen and removes CO}_2$
Answer: digestive and respiratory

Cells release energy through cellular respiration, which requires glucose (supplied by digestion) and oxygen (supplied by respiration).

Question 2 · 2 pts

An example of an activity that best contributes to maintaining homeostasis in an organism is a

Worked solution
$\text{Homeostasis = maintaining stable internal conditions}$
$\text{Immune response to vaccine protects the body, maintaining stability}$
Answer: response to a chickenpox vaccination

Producing antibodies in response to a vaccine helps the body maintain a stable, healthy internal state, unlike the other choices which represent harmful or disrupted conditions.

Question 3 · 2 pts

Equine cloning can be used to produce performance horses. Although the horses are clones of each other, they may still exhibit slight differences in appearance. The differences in the physical characteristics of the cloned horses are most likely the result of

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Worked solution
$\text{Clones share identical DNA}$
$\text{Phenotype differences among genetically identical individuals} \Rightarrow \text{environment}$
Answer: environmental influences

Since the clones have identical genes, any differences in their appearance must come from environmental factors rather than genetic ones.

Question 4 · 2 pts

Which situation is an example of an organism responding to an abiotic factor?

Worked solution
$\text{Abiotic factors: non-living, e.g. sunlight, temperature, water}$
$\text{Growing toward sunlight = response to an abiotic factor}$
Answer: Plants in a forest grow toward areas where there is more sunlight available.

Sunlight is a nonliving (abiotic) factor, so plants growing toward it is a direct response to an abiotic factor, unlike the other choices which involve responses to other organisms (biotic factors).

Question 5 · 2 pts

CRISPR/Cas9 is a powerful system that bacteria use to cut and remove DNA from invading viruses. Using CRISPR/Cas9, researchers have successfully corrected a disease-causing mutation for muscular dystrophy in laboratory mice. Correcting the harmful mutation using CRISPR/Cas9 is an example of

Worked solution
$\text{Directly editing DNA sequences} \Rightarrow \text{genetic engineering}$
Answer: genetic engineering

Directly altering an organism's DNA using a tool like CRISPR/Cas9 to fix a mutation is genetic engineering, not selective breeding, cloning, or natural evolution.

Question 6 · 2 pts

Many animal populations living in a particular area would most likely

Worked solution
$\text{Organisms sharing a habitat need similar temperature, water, oxygen conditions}$
$\text{Competitive exclusion prevents identical niches or food sources}$
Answer: have similar physical requirements

Populations sharing the same area must tolerate similar physical conditions (temperature, water, oxygen), while occupying the same niche or eating identical food would cause direct competition that is not sustainable for both.

Question 7 · 2 pts

Cells possess structures that perform specific jobs. Which statement correctly pairs a cell structure with a function it performs in the cells?

Worked solution
$\text{Mitochondria: site of cellular respiration} \Rightarrow \text{ATP production}$
Answer: The mitochondria provide energy for cell processes.

Mitochondria carry out cellular respiration to release energy (ATP) for the cell; the other pairings describe incorrect functions for those organelles.

Question 8 · 2 pts

The endangered Everglade snail kite is a predatory bird which usually feeds on small snails. Conservationists feared the snail kite would face a greater decline when the Everglades was invaded by a species of larger snail that the birds had historically struggled to eat. But the snail kite population increased over several years, and the snail kites now have larger beaks and bodies. This change in the snail kite can best be explained by

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Worked solution
$\text{New food source (larger snails) = environmental change}$
$\text{Kites with larger beaks survive/reproduce better} \Rightarrow \text{trait increases in population}$
Answer: natural selection after an environmental change

The arrival of larger snails changed the environment, and kites with larger beaks and bodies were better able to survive and reproduce, so this trait became more common through natural selection.

Question 9 · 2 pts

Scientists turned a specialized stomach cell from a mouse into a skin cell by activating a specific gene responsible for the production of skin cells. Which claim can be made, based on this evidence?

Worked solution
$\text{All body cells contain the same full set of genes}$
$\text{Activating a different gene changed cell type} \Rightarrow \text{stomach cell had that gene all along}$
Answer: Stomach cells have the genetic information to form other types of cells.

Since activating a single gene changed a stomach cell into a skin cell, the stomach cell must have already contained the genetic information needed to become a skin cell.

Question 10 · 2 pts

New York State is home to animals such as the Eastern chipmunk. Individuals within this species are not genetically identical. This variability is primarily the result of

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Worked solution
$\text{Meiosis produces genetically varied gametes}$
$\text{Recombination (crossing over, independent assortment) increases variation}$
Answer: meiosis and recombination

Genetic variability among individuals of a sexually reproducing species comes mainly from meiosis and the recombination of genetic material it produces.

Question 11 · 2 pts

Zebra mussels are aquatic animals found in many bodies of fresh water in New York State. These organisms are not native to North America. When these mussels first appeared, their populations increased rapidly, which led scientists to fear their potential impact on native species. Lately, it has been observed that the rate of population growth of the zebra mussels has decreased. A reason for this decrease may be

Worked solution
$\text{Population growth slows as carrying capacity is approached}$
$\text{Limited resources} \Rightarrow \text{slower growth rate}$
Answer: resources needed for the continued growth of their population are limited

As a population grows, limited resources such as food and space cause the growth rate to slow as it approaches the carrying capacity of the environment.

Question 12 · 2 pts

The diagram below represents a food web. Bushes point to Insects and to Rodents. Grasses point to Insects and to Lizards. Rodents point to Coyotes and to Insects. Insects point to Coyotes, to Roadrunners, and to Lizards. Lizards point to Roadrunners. Which statement best describes a relationship represented in the diagram?

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Worked solution
$\text{Arrow from Insects} \rightarrow \text{Roadrunners means insects are eaten by roadrunners}$
$\text{Roadrunners consume animals} \Rightarrow \text{carnivores}$
Answer: Roadrunners are carnivores that feed on insects.

An arrow points from Insects to Roadrunners in the food web, showing that roadrunners are carnivorous consumers that feed on insects.

Question 13 · 2 pts

Cell membranes inside the cells that line the stomach pump hydrogen ions from areas of low concentration inside the cells to areas of higher concentration outside the cells. Which activity produces the ATP that makes this pumping possible?

Worked solution
$\text{Moving ions against a concentration gradient = active transport, requires ATP}$
$\text{ATP is produced by cellular respiration}$
Answer: cellular respiration

Pumping ions against their concentration gradient requires energy in the form of ATP, which is produced through cellular respiration.

Question 14 · 2 pts

If scientists wanted to study the physical characteristics of an extinct animal that once lived in a specific area, the best source of information would be to investigate

Worked solution
$\text{Extinct animal} \Rightarrow \text{no living specimens}$
$\text{Fossils are direct physical evidence of past organisms}$
Answer: the fossil record of that area

Because the animal is extinct, direct evidence of its physical characteristics can only come from fossils, not from currently living organisms.

Question 15 · 2 pts

Tasmanian devils are predators found on the Tasman Peninsula of Australia. Their numbers were greatly reduced after two forms of contagious cancer appeared in the population. Scientists have found an effective cancer vaccine that has saved a number of adult Tasmanian devils. The beneficial effect of the vaccine will not be passed on to the Tasmanian devils' offspring because the

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Worked solution
$\text{Only changes to sex cells (gametes) are heritable}$
$\text{Vaccine affects the immune system, not the DNA of gametes}$
Answer: vaccine did not produce a change in the sex cells of the adults

Traits are only passed to offspring through changes in sex cells; since the vaccine does not alter the DNA of the sex cells, its protective benefit is not inherited.

Question 16 · 2 pts

Usually, snakes reproduce sexually. However, some female copperhead snakes sometimes produce offspring asexually without sperm from a male. Compared with snakes formed by sexual reproduction, the offspring of these asexually reproducing snakes

Worked solution
$\text{Asexual reproduction} \Rightarrow \text{offspring genetically identical (or nearly so) to parent}$
$\text{No mixing of genetic material from two parents} \Rightarrow \text{limited variation}$
Answer: have limited genetic variation

Because asexual reproduction does not combine genetic material from two parents, the offspring have much less genetic variation than offspring produced sexually.

Question 17 · 2 pts

The brown anole is native to Cuba and the Bahamas. Males and females of the species share most of the same genes. They are the same size when they hatch out of their eggs. However, during the first year, the males grow to be three times larger than the females. The most likely explanation for the differences in size between male and female anoles is that

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Worked solution
$\text{Males and females share the same genes}$
$\text{Different size outcomes} \Rightarrow \text{differential gene expression, controlled by hormones}$
Answer: hormones can affect gene expression

Since males and females share nearly identical genes but grow very differently, the difference must result from hormones causing different genes to be expressed (turned on or off), not from different genetic information.

Question 18 · 2 pts

Myasthenia gravis is an autoimmune disease characterized by weakness of the skeletal muscles. It occurs when normal communication between nerve and muscle cells is interrupted. The weakness is likely due to

Worked solution
$\text{Nerve-muscle communication uses chemical signals (neurotransmitters) binding to receptors}$
$\text{Interrupted communication} \Rightarrow \text{receptor malfunction on muscle cells}$
Answer: the failure of receptor molecules on the muscle to receive the chemical produced by nerve cells

Since the disease involves interrupted communication between nerve and muscle cells, the problem lies in the muscle cell receptors failing to properly receive the chemical signal released by nerve cells.

Question 19 · 2 pts

The removal of a short sequence of bases from a gene would most directly affect the

Worked solution
$\text{Gene sequence} \rightarrow \text{mRNA sequence} \rightarrow \text{amino acid sequence} \rightarrow \text{protein shape}$
$\text{Removing bases changes the amino acid sequence} \Rightarrow \text{changed protein shape}$
Answer: shape of a protein molecule

Since genes code for the amino acid sequence of proteins, removing bases from a gene changes that sequence and therefore the resulting protein's shape.

Question 20 · 2 pts

As energy moves through a forest ecosystem, it flows from

Worked solution
$\text{Energy flow: autotrophs (producers)} \rightarrow \text{herbivores} \rightarrow \text{carnivores}$
Answer: herbivores to carnivores

Energy flows one direction through an ecosystem, from producers to herbivores and then to carnivores, never in the reverse direction as in the other choices.

Question 21 · 2 pts

Each winter in the Adirondack Mountains, some of the salt applied to roadways gets washed into lakes. The increase in salt levels in areas where frogs breed has resulted in more male frogs hatching than females. This is an example of

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Worked solution
$\text{Salt (abiotic factor)} \Rightarrow \text{alters which sex genes are expressed during development}$
Answer: an abiotic factor affecting gene expression

The increased salt in the water is a nonliving (abiotic) environmental factor that is influencing which sex-determining genes are expressed, resulting in more males hatching.

Question 22 · 2 pts

Which substances usually stimulate an immune response?

Worked solution
$\text{Antigens are foreign markers recognized by the immune system}$
$\text{Immune system responds by producing antibodies against antigens}$
Answer: antigens

Antigens are foreign substances that the immune system recognizes as invaders, triggering an immune response such as antibody production.

Question 23 · 2 pts

A certain species of rough-skinned newt produces an extremely powerful toxin that helps prevent attacks by predators. However, one predator, the garter snake, can eat these newts without being affected by the toxin. Which statement best explains the resistance of garter snakes to the newt toxin?

Worked solution
$\text{Mutations arise randomly, not because an organism needs them.}$
$\text{Snakes with a pre-existing resistance mutation survived and reproduced more.}$
$\text{This is natural selection: the favorable trait was passed to offspring, increasing its frequency.}$
Answer: A random genetic mutation that resulted in toxin resistance increased the survival rates of the snakes that had it, and they passed it on to their offspring.

This is classic natural selection, resistance did not arise because it was needed (choices 1-3 imply purposeful or use-driven change); rather, a pre-existing random mutation gave some snakes a survival advantage that was then passed on.

Question 24 · 2 pts

The photo below is of a magnified podocyte, a highly specialized cell that produces special proteins for filtering fluid in the human kidney. The specialized function of this cell is most dependent on

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Worked solution
$\text{Specialized proteins for filtering come from gene expression.}$
$\text{DNA codes (genes) are transcribed and translated by ribosomes to make specific proteins.}$
$\text{This protein synthesis pathway, not mutation or organelle structure alone, drives the specialized function.}$
Answer: the DNA codes in the cell and the activity of ribosomes

A cell's specialized function depends on which genes are expressed and translated into proteins by ribosomes, not on mutations or on unrelated organelles like mitochondria.

Question 25 · 2 pts

Maintaining stability in an ecosystem most likely depends on

Worked solution
$\text{Greater biodiversity means more ecological niches are filled.}$
$\text{More niches and species create redundancy and multiple energy pathways.}$
$\text{This redundancy buffers the ecosystem against disturbance, promoting stability.}$
Answer: a high level of diversity and multiple ecological niches

Ecosystem stability is generally associated with high biodiversity and many available niches, which provide redundancy and resilience against disturbances.

Question 26 · 2 pts

Photosynthesis and cellular respiration both involve the gases carbon dioxide and oxygen. Which statement best identifies how these gases are involved in the two processes?

Worked solution
$\text{Photosynthesis: } 6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$
$\text{Cellular respiration: } C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O$
$\text{These two processes use and release opposite gases.}$
Answer: Cellular respiration uses oxygen and releases carbon dioxide, while photosynthesis uses carbon dioxide and releases oxygen.

Photosynthesis consumes carbon dioxide and releases oxygen, while cellular respiration consumes oxygen and releases carbon dioxide, the two processes are complementary.

Question 27 · 2 pts

Antibodies produced against one pathogen infecting the human body may not work against a different pathogen because antibodies are

Worked solution
$\text{Antibodies bind antigens via a lock-and-key shape match.}$
$\text{Each antibody's shape is specific to one antigen's surface proteins.}$
$\text{A different pathogen has differently shaped surface proteins, so the antibody won't bind it.}$
Answer: specific for the shape of the proteins present on a particular pathogen

Antibody-antigen binding is highly specific, based on the complementary shape between the antibody and the particular protein markers on a pathogen's surface.

Question 28 · 2 pts

A multicellular organism has cells that perform various roles in that organism. This is most likely due to the

Worked solution
$\text{A zygote divides repeatedly during embryonic development.}$
$\text{Cells differentiate, turning on different genes, to become specialized cell types.}$
$\text{This differentiation produces the variety of roles cells perform in the organism.}$
Answer: differentiation of cells during embryonic development

Differentiation during embryonic development causes genetically identical cells to take on different structures and functions, producing the variety of specialized cells in a multicellular organism.

Question 29 · 2 pts

The following describes a response that occurs in the guard cells of a plant. When the Sun is shining and a large amount of water is available, the guard cells open the leaf pores. When the Sun is shining but little water is available, the guard cells close the leaf pores. The changes in the guard cells' activity illustrate

Worked solution
$\text{Guard cells respond to internal water availability, not just sunlight.}$
$\text{When water is plentiful, pores open; when water is scarce, pores close.}$
$\text{This self-adjusting response to water status is a feedback mechanism regulating water loss.}$
Answer: a feedback mechanism to control water loss

The opposite guard cell responses to water availability show a feedback mechanism that regulates transpiration (water loss) to maintain homeostasis in the plant.

Question 30 · 2 pts

Today's whales and alligators both have pelvic and hind leg bones, yet these bones only function in alligators. This similarity between whales and alligators supports the idea that

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Worked solution
$\text{Vestigial structures (nonfunctional pelvic/leg bones in whales) point to shared ancestry.}$
$\text{Both groups inherited the genetic blueprint for these bones from a common ancestor.}$
$\text{In whales the structures lost function over evolutionary time; in alligators they remain functional.}$
Answer: alligators and whales share a common ancestor

Vestigial pelvic and hind leg bones in whales are homologous to the functional versions in alligators, evidence that both descended from a common ancestor that had functional leg bones.

Question 31 · 2 pts

Base your answer on the information below. Warming Water Leaves Fish Gasping for Air and Shrinking in Size. As fish grow into adulthood, their body mass increases and so does their demand for oxygen. However, the gills, through which oxygen is obtained, do not increase in size at the same rate as the body. Scientists have observed that as the ocean waters become warmer, there is less dissolved oxygen in the water. The result is that the average size of many fish species becomes smaller. The most likely reason decreased levels of oxygen in the water result in a decrease in the body size of some fish species is

Worked solution
$\text{Gills do not grow at the same rate as body mass.}$
$\text{Warmer water holds less dissolved oxygen, further limiting oxygen intake.}$
$\text{Less oxygen limits cellular respiration/ATP production, so a larger body's energy needs cannot be met, favoring smaller body size.}$
Answer: the species being unable to meet the energy requirements of a larger body size

Because gill surface area cannot keep pace with a growing body's oxygen demand, and warm water further reduces available dissolved oxygen, fish cannot generate enough energy (via cellular respiration) to sustain a larger body size.

Question 32 · 2 pts

One human activity that most directly contributes to the decrease in the amount of oxygen present in ocean water is

Worked solution
$\text{Industrialization releases greenhouse gases (CO}_2\text{) that warm the atmosphere and oceans.}$
$\text{Warmer water holds less dissolved oxygen (solubility of gases decreases with temperature).}$
$\text{This reduces the oxygen available to marine organisms.}$
Answer: industrialization, which releases large amounts of carbon dioxide into the atmosphere

Industrial CO2 emissions drive global warming, and warmer ocean water holds less dissolved oxygen, directly decreasing oxygen availability in ocean water.

Question 33 · 2 pts

The graph below represents the interactions of two female reproductive hormones. Based on the graph, which statement is correct regarding the interaction of the levels of estrogen and progesterone?

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Worked solution
$\text{Egg release occurs at day 14, at the estrogen peak.}$
$\text{At day 14, the solid (estrogen) line is at its highest point while the dashed (progesterone) line is still low.}$
$\text{Thus at ovulation estrogen > progesterone.}$
Answer: When an egg is released from the ovary, the level of estrogen is higher than the level of progesterone.

At the moment of egg release (day 14) the graph shows estrogen at its peak while progesterone is still near its baseline, so estrogen is higher than progesterone at ovulation.

Question 34 · 2 pts

Base your answer on the information below. Fitness of Male Juncos. Animals communicate with each other in many ways. For example, many male birds have bright colors to signal their fitness to females. Scientists hypothesized that female birds also use their sense of smell to gather information about the fitness of their potential mates. To test this, scientists gathered male juncos and determined the amount of a chemical produced by the male birds that is sensed by the female birds. Scientists then collected data on the number of offspring produced by each male during one breeding season. The results are shown in the chart below. Which conclusion is most valid, based on the data?

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Worked solution
$\text{The trend line on the scatter plot has a positive slope.}$
$\text{As \% of male chemical increases along the x-axis, number of offspring (y-axis) generally increases.}$
$\text{This positive correlation supports greater reproductive success at higher chemical percentages.}$
Answer: Male juncos with a higher percentage of the male chemical have greater reproductive success.

The scatter plot's upward trend line shows a positive correlation between percent of male chemical and number of offspring, indicating males producing more of the chemical tend to have greater reproductive success.

Question 35 · 2 pts

Pikas are small mammals found in the grassland ecosystems of the Tibetan plateau. Pikas are prey for many of the predators also inhabiting the Tibetan grasslands, which serves as an important watershed for the area. This watershed drains large amounts of groundwater during the rainy season. Pikas have large burrow systems that help to quickly drain the groundwater. The burrows also serve as nesting sites for numerous bird species. Because they compete with livestock for grass, many people want the pikas totally removed from the Tibetan plateau. If the pika populations are completely removed from the grasslands of the Tibetan plateau, the most likely result will be that the grassland ecosystems will become

Worked solution
$\text{Pikas serve as prey, provide nesting burrows for birds, and drain groundwater via burrows.}$
$\text{Removing pikas removes a food source, eliminates nesting sites, and stops groundwater drainage.}$
$\text{Loss of multiple ecosystem functions destabilizes the ecosystem.}$
Answer: unstable, because predators will have fewer prey, the birds will have fewer nesting sites, and groundwater supplies will be disrupted

Pikas play multiple interconnected ecological roles (prey, burrow-nesting habitat, groundwater drainage), so removing them would disrupt several ecosystem functions simultaneously, causing instability.

Question 36 · 2 pts

Jean-Baptiste Lamarck was a French naturalist who proposed the idea that modern-day organisms developed new characteristics through a process known as the inheritance of acquired traits. As more evidence became available, this theory was eventually replaced by Charles Darwin's theory of evolution. This modification of scientific knowledge illustrates that

Worked solution
$\text{Lamarck's theory was accepted based on the evidence available at the time.}$
$\text{New evidence led scientists to replace it with Darwin's theory.}$
$\text{This shows scientific ideas are provisional and change as new evidence emerges.}$
Answer: all scientific explanations are tentative and subject to change or improvement

The replacement of Lamarck's theory by Darwin's theory as new evidence emerged demonstrates the nature of science: explanations are tentative and can be revised or replaced.

Question 37 · 2 pts

Base your answers to questions 37 through 39 on the information and diagram below. The diagram provides information about some of the digestive enzymes produced by the human pancreas. The activity of nuclease enzymes would most likely result in the release of

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Worked solution
$\text{Nucleases digest nucleic acids (DNA/RNA).}$
$\text{Nucleic acids are polymers of nucleotides built from four nitrogenous bases.}$
$\text{Breaking down nucleic acids releases these molecular bases (subunits).}$
Answer: four different kinds of molecular bases

Nucleases break down nucleic acids, which are made of nucleotides containing one of four nitrogenous bases, so digestion releases these molecular bases.

Question 38 · 2 pts

The end products resulting from the action of amylase would most likely be

Worked solution
$\text{Amylase digests carbohydrates (starches).}$
$\text{Starches are polymers of simple sugar (glucose) subunits.}$
$\text{Digestion by amylase breaks starch down into simple sugars.}$
Answer: simple sugars

Amylase breaks down starch, a carbohydrate polymer, into its simple sugar subunits.

Question 39 · 2 pts

Another important molecule not shown in the diagram is also produced by the pancreas. It functions to decrease glucose levels in the blood. This molecule is

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Worked solution
$\text{The pancreas produces the hormone insulin.}$
$\text{Insulin signals cells to take up glucose from the blood.}$
$\text{This lowers blood glucose levels, maintaining homeostasis.}$
Answer: insulin

Insulin, produced by the pancreas, is the hormone responsible for lowering blood glucose levels by promoting glucose uptake into cells.

Question 40 · 2 pts

The process of meiotic division in human males is represented below. This process produces four sperm cells, each with

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Worked solution
$\text{Meiosis reduces chromosome number by half, from diploid (2n) to haploid (n).}$
$\text{One diploid germ cell undergoes two meiotic divisions to yield four haploid sperm cells.}$
$\text{Each sperm cell has half the genetic information of the original diploid cell.}$
Answer: one-half of the genetic information found in the diploid germ cell

Meiosis is a reduction division that halves the chromosome number, so each of the four resulting sperm cells contains half the genetic information of the original diploid germ cell.

Question 41 · 2 pts

A student viewed a slide of an onion root tip with a compound light microscope. The photograph below represents what he saw. In order to observe whether or not this root tip was growing, the student should

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Worked solution
$\text{Growth in a root tip occurs through mitotic cell division.}$
$\text{Higher magnification allows clearer observation of individual cells and dividing structures (chromosomes).}$
$\text{Evidence of cell division (not just size) confirms growth is occurring.}$
Answer: switch to a higher magnification and look for evidence of cell division

Growth at a root tip results from mitosis, so increasing magnification to look for cells undergoing division provides direct evidence of growth activity.

Question 42 · 2 pts

Which statement is an example of a hypothesis that can be tested through experimentation?

Worked solution
$\text{A testable hypothesis must be a measurable, falsifiable statement, not a question or an unmeasurable/subjective claim.}$
$\text{Choice 1 assumes an unmeasurable emotional link; choice 2 is a question, not a hypothesis; choice 3 anthropomorphizes plants ("fear") which cannot be measured.}$
$\text{"Bacterial growth will rapidly increase as temperature increases" is a measurable, testable statement.}$
Answer: Bacterial growth will rapidly increase as the temperature increases.

A valid testable hypothesis is a declarative, measurable statement about a relationship between variables; only the bacterial growth/temperature statement fits this, the others are a question, an assumption, or an unmeasurable claim.

Question 43 · 2 pts

Deforestation is a major cause of soil loss. Without trees and other plants to hold the soil in place, it either washes or blows away. Governments, international organizations, and others are working to decrease the rate of deforestation. In addition to slowing the rate of soil loss, another potential benefit of this action would be

Worked solution
$\text{Trees remove carbon dioxide from the atmosphere via photosynthesis.}$
$\text{Slowing deforestation preserves more trees performing this function.}$
$\text{More trees photosynthesizing leads to lower atmospheric CO}_2\text{ levels.}$
Answer: a decrease in atmospheric carbon dioxide levels

Preserving forests maintains more trees that absorb carbon dioxide through photosynthesis, which helps decrease atmospheric carbon dioxide levels, an added benefit beyond reducing soil loss.

Question 44 · 1 pt · constructed response

Base your answer on the information and graph below and on your knowledge of biology. The graph shows the amount of oxygen in Earth's atmosphere from 3500 million years ago to present. Scientists can use this information to learn more about the evolution of different species. Identify when during Earth's history autotrophs most likely first appeared. Support your answer using information from the graph.

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Model answer: Autotrophs most likely first appeared around 2500 million years ago (mya), because that is when the graph shows atmospheric oxygen beginning to rise from its previously flat, near-zero level, oxygen release is a byproduct of photosynthesis carried out by autotrophs.
Worked solution
$\text{The graph is flat (very low oxygen) from 3500 mya to about 2500 mya.}$
$\text{Oxygen levels begin increasing starting around 2500 mya.}$
$\text{Since photosynthetic autotrophs release oxygen as a byproduct, the onset of rising oxygen marks their appearance, so autotrophs most likely first appeared around 2500 mya.}$
Answer: Autotrophs most likely first appeared around 2500 million years ago (mya), because that is when the graph shows atmospheric oxygen beginning to rise from its previously flat, near-zero level, oxygen release is a byproduct of photosynthesis carried out by autotrophs.

Because the graph shows atmospheric oxygen was flat and low until about 2500 million years ago and then began rising, and because oxygen is a byproduct of photosynthesis, this rise indicates that photosynthetic autotrophs most likely first appeared around 2500 million years ago.

Question 45 · 1 pt · constructed response

Base your answers to questions 45 through 49 on the information and data table below and on your knowledge of biology. Whitebark Pines in Yellowstone Park Scientists claim that mountain pine beetles have been blamed for the death of mature whitebark pine trees in the Greater Yellowstone forest ecosystem. The beetles burrow into the trees to lay their eggs. When the eggs hatch, the larvae feed on the tree, cutting off the flow of water. As a result, the trees become stressed and begin to die. An increase in the temperature is contributing to the increase in beetles. Cooler temperatures tend to keep the beetle population controlled. Many organisms, including squirrels, birds, and even grizzly bears, have been impacted by the decrease in the numbers of trees. Many organisms use whitebark pine seeds for food. The data table below shows the proportion of live mature whitebark pine trees compared with their population in 2000. Mature Whitebark Pine Trees in the Greater Yellowstone Ecosystem | Year | Proportion of Live Mature Whitebark Pine Trees Compared with Their Population in 2000 | | --- | --- | | 2000 | 1.00 | | 2002 | 1.00 | | 2004 | 0.70 | | 2006 | 0.60 | | 2008 | 0.40 | | 2010 | 0.25 | | 2012 | 0.25 | Directions (45-46): Using the information in the data table, construct a line graph on the grid, following the directions below. 45 Mark an appropriate scale, without any breaks in the data, on each labeled axis.

live question 45 figure
Model answer: x-axis (Year): mark 2000 through 2012 in equal, evenly spaced intervals (e.g., every 2 years) with no breaks. y-axis (Proportion of Live Mature Whitebark Pine Trees): mark 0 through 1.00 in equal increments (e.g., 0.10 per grid line) starting at 0, with no breaks.
Worked solution
$\text{x-axis range: } 2000 \text{ to } 2012, \text{ equal spacing, no breaks}$
$\text{y-axis range: } 0 \text{ to } 1.00, \text{ equal spacing (e.g., } 0.10 \text{ per box), no breaks}$
Answer: x-axis (Year): mark 2000 through 2012 in equal, evenly spaced intervals (e.g., every 2 years) with no breaks. y-axis (Proportion of Live Mature Whitebark Pine Trees): mark 0 through 1.00 in equal increments (e.g., 0.10 per grid line) starting at 0, with no breaks.

An appropriate scale uses equal intervals that span the full range of the data on each axis without skipping or compressing any values (no breaks).

Question 46 · 1 pt · constructed response

Plot the data on the grid. Connect the points and surround each point with a small circle. (Example shown: points connected by a line, with each point circled.)

live question 46 figure
Model answer: Plot and connect the points: (2000, 1.00), (2002, 1.00), (2004, 0.70), (2006, 0.60), (2008, 0.40), (2010, 0.25), (2012, 0.25), circling each plotted point.
Worked solution
$(2000,1.00),\ (2002,1.00),\ (2004,0.70),\ (2006,0.60),\ (2008,0.40),\ (2010,0.25),\ (2012,0.25)$
$\text{Connect consecutive points with straight line segments and circle each point}$
Answer: Plot and connect the points: (2000, 1.00), (2002, 1.00), (2004, 0.70), (2006, 0.60), (2008, 0.40), (2010, 0.25), (2012, 0.25), circling each plotted point.

Each year/proportion pair from the data table is plotted at the correct grid location, the points are connected in sequence with straight lines, and each point is circled as shown in the example.

Question 47 · 2 pts

Note: The answer to question 47 should be recorded on your separate answer sheet. 47 The nutritional roles of the whitebark pine trees and the beetles are best described as

Worked solution
$\text{Whitebark pine trees make their own food (photosynthesis)} \Rightarrow \text{producer}$
$\text{Beetle larvae feed on the living tissue of the tree} \Rightarrow \text{herbivore}$
Answer: producer and herbivore

The pine trees are autotrophic producers, and the beetles consume plant tissue, making them herbivores.

Question 48 · 1 pt · constructed response

Warmer climatic temperatures are one of the reasons for the increase in the pine beetle population. State one action that humans can take to help to reduce this warming trend.

Whitebark Pines in Yellowstone Park Scientists claim that mountain pine beetles have been blamed for the death of mature whitebark pine trees in the Greater Yellowstone forest ecosystem. The beetles burrow into the trees to lay their eggs. When the eggs hatch, the larvae feed on the tree, cutting off the flow of water. As a result, the trees become stressed and begin to die. An increase in the temperature is contributing to the increase in beetles. Cooler temperatures tend to keep the beetle population controlled. Many organisms, including squirrels, birds, and even grizzly bears, have been impacted by the decrease in the numbers of trees. Many organisms use whitebark pine seeds for food. The data table below shows the proportion of live mature whitebark pine trees compared with their population in 2000. Mature Whitebark Pine Trees in the Greater Yellowstone Ecosystem | Year | Proportion of Live Mature Whitebark Pine Trees Compared with Their Population in 2000 | | --- | --- | | 2000 | 1.00 | | 2002 | 1.00 | | 2004 | 0.70 | | 2006 | 0.60 | | 2008 | 0.40 | | 2010 | 0.25 | | 2012 | 0.25 | Directions (45-46): Using the information in the data table, construct a line graph on the grid, following the directions below.

Model answer: Reduce the burning of fossil fuels (e.g., by using renewable energy sources such as solar or wind power, or by driving less/carpooling/using public transportation) to lower carbon dioxide emissions.
Worked solution
$\text{Warming is linked to greenhouse gas (CO}_2\text{) emissions from burning fossil fuels}$
$\text{Reducing fossil fuel use lowers CO}_2\text{ output, slowing the warming trend}$
Answer: Reduce the burning of fossil fuels (e.g., by using renewable energy sources such as solar or wind power, or by driving less/carpooling/using public transportation) to lower carbon dioxide emissions.

Any specific human action that reduces greenhouse gas emissions, such as switching to renewable energy or reducing fossil fuel combustion, is acceptable.

Question 49 · 2 pts

Note: The answer to question 49 should be recorded on your separate answer sheet. 49 The dependent variable in this study is the

Whitebark Pines in Yellowstone Park Scientists claim that mountain pine beetles have been blamed for the death of mature whitebark pine trees in the Greater Yellowstone forest ecosystem. The beetles burrow into the trees to lay their eggs. When the eggs hatch, the larvae feed on the tree, cutting off the flow of water. As a result, the trees become stressed and begin to die. An increase in the temperature is contributing to the increase in beetles. Cooler temperatures tend to keep the beetle population controlled. Many organisms, including squirrels, birds, and even grizzly bears, have been impacted by the decrease in the numbers of trees. Many organisms use whitebark pine seeds for food. The data table below shows the proportion of live mature whitebark pine trees compared with their population in 2000. Mature Whitebark Pine Trees in the Greater Yellowstone Ecosystem | Year | Proportion of Live Mature Whitebark Pine Trees Compared with Their Population in 2000 | | --- | --- | | 2000 | 1.00 | | 2002 | 1.00 | | 2004 | 0.70 | | 2006 | 0.60 | | 2008 | 0.40 | | 2010 | 0.25 | | 2012 | 0.25 | Directions (45-46): Using the information in the data table, construct a line graph on the grid, following the directions below.

Worked solution
$\text{Independent variable (what is manipulated/tracked): time (year)}$
$\text{Dependent variable (what is measured in response): proportion of live mature whitebark pine trees}$
Answer: proportion of live mature whitebark pine trees

The dependent variable is the measured outcome that changes in response to time, which here is the proportion of live mature whitebark pine trees.

Question 50 · 2 pts

Base your answers to questions 50 and 51 on the information below and on your knowledge of biology. Pepsin is a protein-digesting enzyme. It is produced inside cells that line the stomach and then secreted into the stomach cavity, where it begins to work. When first produced, the pepsin exists in an inactive form called pepsinogen. Pepsinogen cannot work because it has an extra segment that keeps it from interacting with the proteins it would normally digest. When it is secreted into the stomach cavity, the acid there causes the pepsinogen molecule to lose this extra segment, which changes it into the active pepsin that can begin to digest food proteins. Note: The answer to question 50 should be recorded on your separate answer sheet. 50 Which statement most accurately summarizes the function of pepsin?

Worked solution
$\text{Pepsin is an enzyme (biological catalyst)}$
$\text{Enzymes speed up/control the rate of specific chemical reactions, here protein digestion}$
Answer: It controls the rate at which certain chemical reactions occur.

As an enzyme, pepsin's function is to catalyze (control the rate of) the chemical reaction that breaks down proteins.

Question 51 · 1 pt · constructed response

Explain why the extra segment prevents pepsinogen from interacting with food proteins.

Pepsin is a protein-digesting enzyme. It is produced inside cells that line the stomach and then secreted into the stomach cavity, where it begins to work. When first produced, the pepsin exists in an inactive form called pepsinogen. Pepsinogen cannot work because it has an extra segment that keeps it from interacting with the proteins it would normally digest. When it is secreted into the stomach cavity, the acid there causes the pepsinogen molecule to lose this extra segment, which changes it into the active pepsin that can begin to digest food proteins.

Model answer: The extra segment blocks or covers the active site of the molecule, changing its shape so that food protein molecules cannot bind to (fit into) it.
Worked solution
$\text{Enzyme activity requires the substrate to bind the active site}$
$\text{Extra segment covers/alters the active site} \Rightarrow \text{shape no longer complementary to substrate} \Rightarrow \text{no binding/reaction}$
Answer: The extra segment blocks or covers the active site of the molecule, changing its shape so that food protein molecules cannot bind to (fit into) it.

The extra segment changes the three-dimensional shape of the molecule so the active site is blocked, preventing the substrate (food protein) from binding.

Question 52 · 1 pt · constructed response

Base your answers to questions 52 and 53 on the information below and on your knowledge of biology. Trixie's Pedigree Pet owners today have access to genetic technologies that can provide them with information about their pets. For example, ancestry charts (pedigrees) can be developed for dogs by analyzing specific DNA sequences that are present in their cells. The presence of these DNA sequences can be used to determine the types of breeds present in the dogs' ancestors. The chart represents the family tree of a dog named Trixie, showing her great-grandparents, grandparents, and parents (a Pekingese/Mixed Breed line and an Australian Shepherd line), resulting in Trixie from a Pekingese Mix crossed with an Australian Shepherd. 52 Explain why it only requires a sample of cheek cells present in the dog's saliva, rather than using a mix of cells present in different tissues of the dog, to determine the breeds making up the dog's ancestry.

live question 52 figure
Model answer: Every cell in the dog's body (including cheek cells) contains the same complete set of DNA/genetic information, produced by mitosis from the original fertilized egg, so a sample from any one tissue is sufficient to reveal the dog's ancestry.
Worked solution
$\text{All body cells arise from mitosis of the same zygote}$
$\Rightarrow \text{all cells (cheek cells or otherwise) contain identical DNA}$
Answer: Every cell in the dog's body (including cheek cells) contains the same complete set of DNA/genetic information, produced by mitosis from the original fertilized egg, so a sample from any one tissue is sufficient to reveal the dog's ancestry.

Because mitosis produces genetically identical cells throughout the body, DNA from cheek cells is the same as DNA in any other tissue, making a single sample sufficient.

Question 53 · 1 pt · constructed response

State one possible reason why Trixie could express a trait that was not expressed by any of her ancestors.

Model answer: Trixie could have inherited a recessive allele from each parent (each parent being a carrier without expressing the trait), so the trait only becomes expressed when both recessive alleles are combined; alternatively, a new mutation could have arisen.
Worked solution
$\text{Ancestors could be heterozygous (carriers) for a recessive allele without expressing the trait}$
$\text{Trixie receives the recessive allele from both parents} \Rightarrow \text{homozygous recessive} \Rightarrow \text{trait expressed}$
Answer: Trixie could have inherited a recessive allele from each parent (each parent being a carrier without expressing the trait), so the trait only becomes expressed when both recessive alleles are combined; alternatively, a new mutation could have arisen.

A trait can appear in an offspring for the first time if it is recessive and hidden (masked) in heterozygous ancestors, or due to a new mutation.

Question 54 · 1 pt · constructed response

Base your answers to questions 54 and 55 on the information below and on your knowledge of biology. Biobag System After decades of research, scientists have developed a biobag system with the potential to save extremely premature babies. They have successfully removed eight lamb fetuses from their mothers and placed them into biobags. Eventually, the fetuses developed into healthy sheep. The biobag is a clear plastic bag filled with a solution of water containing various salts. A machine outside the bag is attached to the blood vessels in the lamb's umbilical cord. The lamb's umbilical cord brings in nutrients, and its heart pumps blood through an external oxygenator that removes carbon dioxide from the blood and adds oxygen. The biobag models early development in the reproduction of mammals. In the future, this system may be able to be used with human premature babies. The biobag system could allow them to continue to develop for a longer period of time. 54 Two parts of the biobag system are the plastic bag and the external oxygenator. Select one of these parts and circle it below. Identify the structure in the reproductive system that the part of the biobag system you selected represents, and state the function of that part. Circle one: Plastic bag / External oxygenator

live question 54 figure
Model answer: External oxygenator represents the placenta; its function is to allow exchange of gases and nutrients (oxygen and carbon dioxide, and nutrients/wastes) between the mother's blood and the fetus's blood.
Worked solution
$\text{External oxygenator adds O}_2\text{ and removes CO}_2\text{ from fetal blood}$
$\Rightarrow \text{this is the function performed by the placenta in normal pregnancy}$
Answer: External oxygenator represents the placenta; its function is to allow exchange of gases and nutrients (oxygen and carbon dioxide, and nutrients/wastes) between the mother's blood and the fetus's blood.

The external oxygenator performs gas exchange for the fetus, the same role played by the placenta, which exchanges oxygen, carbon dioxide, nutrients, and wastes between mother and fetus. (Alternatively, the plastic bag represents the amniotic sac/amnion, which contains fluid that cushions and protects the developing fetus.)

Question 55 · 1 pt · constructed response

State one reason why medical advances that might be helpful to people are tested first on organisms such as sheep.

Model answer: Testing on animals such as sheep first helps determine whether the treatment is safe and effective before risking harm to humans.
Worked solution
$\text{Unknown risks of new medical technology}$
$\Rightarrow \text{test on similar mammals first to check safety/effectiveness, avoiding harm to humans}$
Answer: Testing on animals such as sheep first helps determine whether the treatment is safe and effective before risking harm to humans.

Animal testing allows researchers to evaluate safety and effectiveness of a new procedure without exposing humans to unknown risks.

Question 56 · 1 pt · constructed response

Part C Base your answers to questions 56 through 58 on the information below and on your knowledge of biology. Acid Rain Acid rain comes in many forms: wet deposition, such as rain, snow, sleet, hail, and fog, and dry deposition, such as deposits of acid particles, aerosols, and gases. It is formed when sulfur dioxide (SO2) and nitrogen oxides (NOx) combine with moisture in the atmosphere to produce sulfuric acid and nitric acid. Damage to aquatic and forest ecosystems, serious human illness, and the slow destruction of buildings and bridges have all been linked to acid rain. Two sources that contribute to producing acid rain include: - Emissions from airplanes, automobiles, and industries - Emissions of SO2 and NOx from power plants 56 Explain why a change in the pH of lakes and forests as a result of acid rain can upset the dynamic equilibrium of these ecosystems.

Model answer: Organisms in lakes and forests are adapted to survive within a narrow, specific pH range; a change in pH can disrupt enzyme function and chemical reactions in these organisms, causing them to die or fail to reproduce, which disturbs the balance among populations in the ecosystem.
Worked solution
$\text{Organisms adapted to narrow pH range}$
$\text{pH shift} \Rightarrow \text{enzymes/proteins malfunction} \Rightarrow \text{organisms die or reproduce less}$
$\Rightarrow \text{population balance (dynamic equilibrium) disrupted}$
Answer: Organisms in lakes and forests are adapted to survive within a narrow, specific pH range; a change in pH can disrupt enzyme function and chemical reactions in these organisms, causing them to die or fail to reproduce, which disturbs the balance among populations in the ecosystem.

A pH change beyond the tolerance range of organisms interferes with their normal life processes, disturbing the balance of populations that make up the ecosystem's dynamic equilibrium.

Question 57 · 1 pt · constructed response

Identify one specific action individuals could take to significantly reduce the amount of acid rain falling in New York State.

Model answer: Individuals could carpool, use public transportation, or drive less/use fuel-efficient or electric vehicles to reduce emissions from automobiles.
Worked solution
$\text{Reducing fossil fuel combustion by individuals lowers SO}_2\text{/NO}_x\text{ emissions}$
Answer: Individuals could carpool, use public transportation, or drive less/use fuel-efficient or electric vehicles to reduce emissions from automobiles.

Any specific individual action that reduces the burning of fossil fuels (e.g., carpooling, using public transit, using renewable energy) is acceptable.

Question 58 · 1 pt · constructed response

Explain how the specific action you identified in question 57 would decrease the formation of acid rain.

Model answer: Carpooling (or using public transportation) reduces the number of vehicles burning fossil fuels, which decreases the amount of SO2 and NOx emitted into the atmosphere, reducing the amount of these gases available to combine with moisture and form acid rain.
Worked solution
$\text{Fewer vehicles/less fuel burned} \Rightarrow \text{less SO}_2 \text{ and NO}_x \text{ released}$
$\Rightarrow \text{less sulfuric/nitric acid formed in atmosphere} \Rightarrow \text{less acid rain}$
Answer: Carpooling (or using public transportation) reduces the number of vehicles burning fossil fuels, which decreases the amount of SO2 and NOx emitted into the atmosphere, reducing the amount of these gases available to combine with moisture and form acid rain.

Reducing fossil fuel combustion directly lowers the emissions of the gases that combine with atmospheric moisture to create acid rain.

Question 59 · 1 pt · constructed response

Base your answers to questions 59 through 61 on the information below and on your knowledge of biology. Lionfish Invasion Lionfish are native to the Indian and Pacific Oceans. They have recently been found along the southeast coast of the U.S., the Caribbean, and in parts of the Gulf of Mexico. Experts speculate that the lionfish invasion was caused by people dumping unwanted lionfish into the Atlantic Ocean from home aquariums. Lionfish have venomous spines and feed on small crustaceans and many fish, including the young of important commercial fish species such as snapper and grouper. The current invasion of lionfish most likely started with the dumping of about 12 fish. Today, there are thousands of them over a wide area. 59 State one specific reason why these invasive fish have been able to rapidly increase their population and range over the last 20 years.

Model answer: Lionfish have no natural predators in their new environment (the Atlantic), allowing their population to grow unchecked.
Worked solution
$\text{New environment lacks predators/competitors that normally control lionfish numbers}$
$\Rightarrow \text{reduced death rate} \Rightarrow \text{rapid population growth}$
Answer: Lionfish have no natural predators in their new environment (the Atlantic), allowing their population to grow unchecked.

Because lionfish are not native, predators in the Atlantic do not recognize or hunt them, removing a natural check on their population growth. (Abundant food/prey and lack of competition are also acceptable reasons.)

Question 60 · 1 pt · constructed response

Explain why the amount of genetic diversity within the invasive lionfish population would be expected to be quite low.

Model answer: The invasive population began from a very small number of founding individuals (about 12 fish), so the current population descended from a limited gene pool, resulting in low genetic diversity (a founder effect).
Worked solution
$\text{Small founding population (}\approx 12\text{ fish)} \Rightarrow \text{limited gene pool/alleles}$
$\Rightarrow \text{all descendants share a narrow range of genetic variation}$
Answer: The invasive population began from a very small number of founding individuals (about 12 fish), so the current population descended from a limited gene pool, resulting in low genetic diversity (a founder effect).

Starting from only about 12 individuals means the entire invasive population's DNA traces back to a small gene pool, so there is little genetic variation among them.

Question 61 · 1 pt · constructed response

State two ways invasive species can disrupt ecosystems.

Model answer: Two acceptable ways: (1) Invasive species compete with native species for limited resources such as food and space, reducing native population sizes. (2) Invasive species may prey on native species that have no natural defenses against them, reducing or eliminating native populations.
Worked solution
$\text{Competition for resources with native species}$
$\text{Predation on native species lacking defenses/adaptations}$
Answer: Two acceptable ways: (1) Invasive species compete with native species for limited resources such as food and space, reducing native population sizes. (2) Invasive species may prey on native species that have no natural defenses against them, reducing or eliminating native populations.

Invasive species disrupt ecosystems by outcompeting native organisms for resources and/or by preying on native species that are not adapted to defend against them; introducing disease or altering habitats are also acceptable answers.

Question 62 · 1 pt · constructed response

Base your answers to questions 62 through 64 on the information below and on your knowledge of biology. New Drug "Wakes up" Immune System to Fight Pancreatic Cancer Pancreatic cancer makes up three percent of all cancers. Recently, scientists announced the discovery of a new drug that has helped extend the lives of some patients with pancreatic cancer. Pancreatic tumors usually have a protective protein layer surrounding them. The protein is produced by the tumor cells. This protective protein shield seems to deactivate white blood cells that would normally recognize and target the tumor cells for destruction. The new drug reactivates these white blood cells, stimulating them to attack the tumor again. A specific chemotherapy drug blows holes in the protective protein layer around the tumor. This action then allows the activated white blood cells to directly attack the tumor. 62 State whether the new drug used without the chemotherapy drug would enable the immune system to successfully attack the cancer cells. Support your answer.

Model answer: No, the new drug alone would not enable a successful attack, because the protective protein shield around the tumor would remain intact, physically blocking the reactivated white blood cells from reaching and attacking the tumor cells.
Worked solution
$\text{New drug reactivates WBCs but does not remove the protein shield}$
$\text{Shield blocks WBCs from reaching tumor} \Rightarrow \text{no successful attack without chemo drug}$
Answer: No, the new drug alone would not enable a successful attack, because the protective protein shield around the tumor would remain intact, physically blocking the reactivated white blood cells from reaching and attacking the tumor cells.

Since the protein shield still blocks access to the tumor without the chemotherapy drug that creates holes in it, reactivated white blood cells alone cannot reach and destroy the cancer cells.

Question 63 · 1 pt · constructed response

Explain the role of white blood cells in the process of killing cancer cells.

Model answer: White blood cells recognize the tumor cells as abnormal/foreign once reactivated and the protein shield is breached, and they directly attack and destroy the tumor cells.
Worked solution
$\text{Reactivated WBCs recognize tumor cells through gaps in the shield}$
$\Rightarrow \text{WBCs directly attack/destroy the tumor cells}$
Answer: White blood cells recognize the tumor cells as abnormal/foreign once reactivated and the protein shield is breached, and they directly attack and destroy the tumor cells.

White blood cells are part of the immune system's defense that recognizes and destroys abnormal cells such as tumor cells once they can access them.

Question 64 · 1 pt · constructed response

Explain why this new cancer treatment would most likely be less effective if the cancer patient also had AIDS.

New Drug "Wakes up" Immune System to Fight Pancreatic Cancer Pancreatic cancer makes up three percent of all cancers. Recently, scientists announced the discovery of a new drug that has helped extend the lives of some patients with pancreatic cancer. Pancreatic tumors usually have a protective protein layer surrounding them. The protein is produced by the tumor cells. This protective protein shield seems to deactivate white blood cells that would normally recognize and target the tumor cells for destruction. The new drug reactivates these white blood cells, stimulating them to attack the tumor again. A specific chemotherapy drug blows holes in the protective protein layer around the tumor. This action then allows the activated white blood cells to directly attack the tumor.

Model answer: AIDS destroys/weakens the immune system by reducing the number of functioning white blood cells, so there would not be enough active white blood cells available to attack the tumor cells even after the drug treatment.
Worked solution
$\text{AIDS} \Rightarrow \text{destruction of white blood cells (immune cells)}$
$\Rightarrow \text{fewer WBCs available to attack tumor} \Rightarrow \text{treatment less effective}$
Answer: AIDS destroys/weakens the immune system by reducing the number of functioning white blood cells, so there would not be enough active white blood cells available to attack the tumor cells even after the drug treatment.

Because the treatment relies on white blood cells to destroy the tumor, a weakened immune system (as in AIDS) with fewer functioning white blood cells would make the treatment less effective.

Question 65 · 1 pt · constructed response

Base your answers to questions 65 through 67 on the information below and on your knowledge of biology. The Cougars of Zion National Park Researchers claim that the crowds of visitors at Utah's Zion National Park have led to the displacement of cougars, the area's top predator, resulting in a devastating series of changes to the region's biodiversity. The researchers compared Zion Canyon's ecosystem with a nearby habitat called North Creek, where human visits are infrequent and the cougars still thrive. In Zion Canyon, there are many more deer, the cougar's main prey, and fewer cottonwood trees than in North Creek. Zion also has a reduced number and diversity of butterflies, amphibians, and wetland plants. To measure the impact of the shrinking cougar population, researchers collected data on Zion Canyon's deer populations dating back to the 1930s, when tourism began to increase. Currently, with over three million visitors per year, the cougars, which usually avoid humans, are becoming increasingly rare. The researchers also estimated the age and abundance of cottonwoods, a favorite food of young deer, and found a healthy mix of old and young cottonwoods in North Creek, where cougars are common. 65 Students drew a few models of the food chain in Zion National Park, as described in the reading. A: Cottonwoods -> Deer -> Cougar B: Deer -> Cottonwoods -> Cougar C: Cougar -> Deer -> Cottonwoods Record the letter of the model that represents the actual food chain in Zion National Park and explain why the model you chose is correct.

live question 65 figure
Model answer: Model A (Cottonwoods -> Deer -> Cougar) is correct because cottonwoods are producers that make their own food, deer are primary consumers/herbivores that eat cottonwoods (young cottonwoods are a favorite food of deer), and cougars are secondary consumers/predators that eat the deer.
Worked solution
$\text{Cottonwoods (producer)} \rightarrow \text{Deer (primary consumer, eats cottonwoods)} \rightarrow \text{Cougar (predator, eats deer)}$
Answer: Model A (Cottonwoods -> Deer -> Cougar) is correct because cottonwoods are producers that make their own food, deer are primary consumers/herbivores that eat cottonwoods (young cottonwoods are a favorite food of deer), and cougars are secondary consumers/predators that eat the deer.

Energy flows from producer (cottonwoods) to herbivore (deer) to predator (cougar), matching model A and the roles described in the passage.

Question 66 · 1 pt · constructed response

Destabilization is a phenomenon that has been observed in a variety of ecosystems when the number of predators has been significantly reduced. Explain how the destabilization caused by the loss of cougars in Zion National Park has resulted in a decrease in the cottonwood trees.

Model answer: With fewer cougars, there is less predation on deer, so the deer population increases; the larger deer population overgrazes/eats more young cottonwood trees (seedlings/saplings), preventing them from maturing and decreasing the overall number of cottonwood trees.
Worked solution
$\text{Fewer cougars} \Rightarrow \text{less predation pressure on deer}$
$\Rightarrow \text{deer population increases}$
$\Rightarrow \text{more deer feeding on young cottonwoods} \Rightarrow \text{fewer cottonwood trees survive to maturity}$
Answer: With fewer cougars, there is less predation on deer, so the deer population increases; the larger deer population overgrazes/eats more young cottonwood trees (seedlings/saplings), preventing them from maturing and decreasing the overall number of cottonwood trees.

Removing the top predator allows the prey (deer) population to grow unchecked, and the increased deer herbivory on young cottonwoods reduces the cottonwood population, illustrating a trophic cascade.

Question 67 · 1 pt · constructed response

Base your answers on the following: 'The Cougars of Zion National Park.' Researchers claim that the crowds of visitors at Utah's Zion National Park have led to the displacement of cougars, the area's top predator, resulting in a devastating series of changes to the region's biodiversity. The researchers compared Zion Canyon's ecosystem with a nearby habitat called North Creek, where human visits are infrequent and the cougars still thrive. In Zion Canyon, there are many more deer, the cougar's main prey, and fewer cottonwood trees than in North Creek. Zion also has a reduced number and diversity of butterflies, amphibians, and wetland plants. To measure the impact of the shrinking cougar population, researchers collected data on Zion Canyon's deer populations dating back to the 1930s, when tourism began to increase. Currently, with over three million visitors per year, the cougars, which usually avoid humans, are becoming increasingly rare. The researchers also estimated the age and abundance of cottonwoods, a favorite food of young deer, and found a healthy mix of old and young cottonwoods in North Creek, where cougars are common. 67 The researchers claimed the decrease in the cougar population is a result of the increase in the number of visitors in the park. Describe evidence the researchers could use to support their claim.

live question 67 figure
Model answer: Data showing that as the number of park visitors increased over time (since the 1930s), the cougar population/sightings decreased while the deer population increased, and that North Creek (with few visitors) has a stable cougar population and a healthy mix of cottonwood ages, unlike Zion Canyon.
Worked solution
$\text{Compare Zion Canyon (high visitors) to North Creek (low visitors)}$
$\text{Zion Canyon: fewer cougars, more deer, fewer cottonwoods}$
$\text{North Creek: cougars thrive, healthy mix of old/young cottonwoods}$
$\text{Long-term data since the 1930s links rising visitor numbers to declining cougar presence}$
Answer: Data showing that as the number of park visitors increased over time (since the 1930s), the cougar population/sightings decreased while the deer population increased, and that North Creek (with few visitors) has a stable cougar population and a healthy mix of cottonwood ages, unlike Zion Canyon.

A valid piece of supporting evidence is the correlation between rising visitor numbers over decades and the decline in cougar numbers, contrasted with the low-visitor North Creek site where cougars still thrive.

Question 68 · 1 pt · constructed response

Base your answers on the following: 'Meet the Sentinels.' Canaries are the most familiar example of a sentinel species, which are animals and plants that serve as harbingers [indicators] of danger to human health and the environment. In the case of canaries, if odorless carbon monoxide were present in a high enough concentration in a coal mine, the small bird would die first and give miners time to escape. Cats, too, have been sentinels. In the 1950s, people in the town of Minamata, Japan, began to notice that local cats were acting strangely; the cats were unable to walk straight and uncontrollably jumped about. After some time, people began to act similarly. The cause of the 'dancing cat fever' was quickly connected to the release of methylmercury in the wastewater of a local chemical factory. The discharge fed into the city's harbor, where it bioaccumulated in [tissues of] fish and shellfish. Although several thousand people were affected with what became known as Minamata disease, the outcome could have been worse were it not for the warning from the dancing cats. 68 Bald eagle populations declined when a pesticide called DDT was used to kill insects. Once DDT was banned in 1972, the population of bald eagles rebounded. State one reason why the bald eagles could be considered a sentinel species like canaries in coal mines.

Model answer: Bald eagles, as top predators, accumulated high levels of DDT through biomagnification, and their population decline served as an early warning sign of the pesticide's harmful effects on the environment, just as the canaries warned miners of dangerous gas.
Worked solution
$\text{DDT biomagnified up the food chain into eagles}$
$\text{Eagle population decline signaled environmental contamination}$
$\text{This mirrors how canary deaths signaled toxic gas to miners}$
Answer: Bald eagles, as top predators, accumulated high levels of DDT through biomagnification, and their population decline served as an early warning sign of the pesticide's harmful effects on the environment, just as the canaries warned miners of dangerous gas.

A sentinel species reveals hidden environmental danger through its own decline; bald eagles' population crash revealed the toxic effects of DDT before it was widely recognized as harmful to humans.

Question 69 · 1 pt · constructed response

Explain why the banning of certain pesticides might cause a problem for humans.

Model answer: Without the pesticide, populations of disease-carrying or crop-destroying insects (such as mosquitoes or agricultural pests) could increase, leading to a rise in insect-borne disease or a loss of food crops.
Worked solution
$\text{Pesticide banned} \Rightarrow \text{insect population no longer controlled}$
$\text{Insects may spread disease (e.g., malaria) or destroy crops}$
$\text{This creates a new problem for human health or food supply}$
Answer: Without the pesticide, populations of disease-carrying or crop-destroying insects (such as mosquitoes or agricultural pests) could increase, leading to a rise in insect-borne disease or a loss of food crops.

Pesticides, despite their environmental harm, often control organisms that threaten human health or agriculture, so removing them can allow those problems to resurface.

Question 70 · 1 pt · constructed response

Describe one specific human action, other than banning its use, that could reduce the chance that a toxic chemical could contaminate the environment.

Model answer: Properly treat or dispose of the chemical waste (e.g., use water treatment facilities to remove/neutralize methylmercury) before it is released into rivers, harbors, or the environment.
Worked solution
$\text{Identify a toxic chemical's point of release (factory wastewater)}$
$\text{Treat/filter the waste before discharge, or store and dispose of it safely}$
$\text{This prevents the chemical from entering ecosystems and bioaccumulating}$
Answer: Properly treat or dispose of the chemical waste (e.g., use water treatment facilities to remove/neutralize methylmercury) before it is released into rivers, harbors, or the environment.

Proper treatment and containment of industrial waste before disposal keeps toxic substances out of ecosystems, reducing bioaccumulation risk.

Question 71 · 1 pt · constructed response

Base your answers on the following: 'Dinosaur Extinction.' The hypothesis that an asteroid strike led to the mass extinction of dinosaurs is widely accepted. It is understood that the asteroid strike caused a massive and quick shift in Earth's temperature and blocked much of the sunlight. While devastating for the dinosaurs, this dramatic event provided opportunities for other species. For example, surviving birds and mammals underwent a time of rapid evolution, which led to the thousands of birds and mammal species present on Earth today. 71 Describe one way the temporary blocking of the sunlight could have impacted the survival of the dinosaurs.

live question 71 figure
Model answer: Blocking sunlight reduced photosynthesis in plants, causing plants to die off, which removed the food source for herbivorous dinosaurs, and in turn removed the food source (prey) for carnivorous dinosaurs, collapsing the food chain.
Worked solution
$\text{Sunlight blocked} \Rightarrow \text{photosynthesis decreases}$
$\text{Plants die} \Rightarrow \text{herbivores starve}$
$\text{Herbivores die} \Rightarrow \text{carnivores lose food source}$
Answer: Blocking sunlight reduced photosynthesis in plants, causing plants to die off, which removed the food source for herbivorous dinosaurs, and in turn removed the food source (prey) for carnivorous dinosaurs, collapsing the food chain.

Blocked sunlight disrupted the base of the food chain (producers), causing a cascade of starvation up through herbivores and carnivores, ultimately dooming the dinosaurs.

Question 72 · 1 pt · constructed response

Suggest one possible explanation for why some birds were able to survive the mass extinction.

Model answer: Some birds may have had genetic variations, such as small body size or the ability to eat a wide variety of foods (seeds, insects), that allowed them to survive on limited resources during and after the temperature shift and loss of sunlight.
Worked solution
$\text{Variation exists within bird populations}$
$\text{Traits like small size or varied diet increase survival odds in low-food conditions}$
$\text{Birds with these favorable traits survived and reproduced (natural selection)}$
Answer: Some birds may have had genetic variations, such as small body size or the ability to eat a wide variety of foods (seeds, insects), that allowed them to survive on limited resources during and after the temperature shift and loss of sunlight.

Genetic variation meant some birds already possessed traits, such as generalist diets or small size requiring less food, that allowed them to survive the harsh post-asteroid conditions.

Question 73 · 2 pts

Base your answer on the diagram of Darwin's finches, which shows finch species grouped by beak type (probing, grasping, crushing beaks) and feeding habit (insect eaters, seed eaters, fruit eaters, ground insects). Note: The answer to question 73 should be recorded on your separate answer sheet. 73 Which two species of finch would be affected if a bird with a grasping beak that ate ants and beetles was introduced into their habitat?

live question 73 figure
Worked solution
$\text{New bird has grasping beak, eats ants/beetles (insects)}$
$\text{Grasping-beak insect-eating finches: large tree finch, small tree finch}$
$\text{These species share the same niche} \Rightarrow \text{direct competition}$
Answer: large tree finch and small tree finch

The large tree finch and small tree finch both have grasping beaks and eat insects, placing them in direct competition with a newly introduced grasping-beaked, insect-eating bird for the same food resource.

Question 74 · 2 pts

Note: The answer to question 74 should be recorded on your separate answer sheet. 74 Comparisons between species can be done using both structural and molecular evidence. An example of using structural evidence is comparing

Worked solution
$\text{Structural evidence} = \text{physical/anatomical features}$
$\text{Seed characteristics are physical/observable traits}$
$\text{Enzymes, amino acids, DNA banding are molecular evidence}$
Answer: seed characteristics

Structural evidence relies on comparing physical/anatomical traits, such as seed characteristics, while enzyme, amino acid, and DNA comparisons are molecular evidence.

Question 75 · 2 pts

The DNA from three different bacterial species are compared with a specific strain of E. coli bacteria. These data were used to construct the evolutionary tree below, which shows Species G branching off first (most distant), then Species R, then Species P branching closest to E. coli. Which row in the data table below best supports the evolutionary diagram constructed by the scientists?

live question 75 figure
Worked solution
$\text{Tree order (most to least distant from E. coli): } G, R, P$
$\text{Most distant species should share the LEAST \% identical DNA with E. coli}$
$\text{Need } \%G < \%R < \%P$
$\text{Row (2): } 93\% < 95\% < 99\% \checkmark$
Answer: Row (2): E. coli 100%, G 93%, R 95%, P 99%

Since the tree shows G diverging first (most distantly related) and P diverging last (most closely related to E. coli), the percent identical DNA must increase from G to R to P, which only Row (2) satisfies.

Question 76 · 2 pts

Base your answers on the following: A student conducted an experiment to determine the effect of exercising on breathing rate. The student measured the breathing rate of three classmates at rest and again after exercising for intervals of 30, 60, and 90 seconds. Breathing Rate in Breaths/Minute | Time Exercising (seconds) | Student A | Student B | Student C | Average | | --- | --- | --- | --- | --- | | 0 (Resting) | 12 | 12 | 15 | ___ | | 30 | 25 | 18 | 20 | 21 | | 60 | 38 | 27 | 28 | 31 | | 90 | 43 | 33 | 38 | 38 | Note: The answer to question 76 should be recorded on your separate answer sheet. 76 The purpose of checking the breathing rate before exercising is that it

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Worked solution
$\text{Resting rate} = \text{baseline measurement}$
$\text{Baseline is used as the control to compare against exercise results}$
Answer: serves as a control for the experiment

The resting breathing rate acts as the control, providing a baseline against which the effect of the independent variable (exercise) can be measured.

Question 77 · 1 pt · constructed response

Calculate the average resting breathing rate for this group of students. Record your answer in the appropriate space in the data table above.

live question 77 figure
Model answer: 13 breaths/minute
Worked solution
$\text{Average} = \frac{12+12+15}{3}$
$= \frac{39}{3} = 13$
Answer: 13 breaths/minute

Adding the three resting rates (12, 12, 15) and dividing by 3 gives an average resting breathing rate of 13 breaths per minute.

Question 78 · 1 pt · constructed response

State one biological benefit of the breathing rate increasing while exercising.

Model answer: An increased breathing rate delivers more oxygen to muscle cells for cellular respiration (ATP production) and removes carbon dioxide waste more quickly, helping maintain homeostasis during increased activity.
Worked solution
$\text{Exercise} \Rightarrow \text{muscles need more energy (ATP)}$
$\text{More O}_2 \text{ needed for cellular respiration; more CO}_2\text{ produced}$
$\text{Increased breathing rate supplies O}_2\text{ and removes CO}_2\text{ faster}$
Answer: An increased breathing rate delivers more oxygen to muscle cells for cellular respiration (ATP production) and removes carbon dioxide waste more quickly, helping maintain homeostasis during increased activity.

Faster breathing increases oxygen delivery for cellular respiration and speeds carbon dioxide removal, both needed to meet the muscles' higher energy demand during exercise.

Question 79 · 1 pt · constructed response

Base your answers on the following: Three potato slices with the same mass were each placed in three beakers, each labeled with its number and contents. After 30 minutes, the potato slices were removed from the solutions, dried with a paper towel, and the mass was determined. The results are shown in the table below. 79 Identify a process that caused these changes in mass of each of the three slices.

live question 79 figure
Model answer: Osmosis (the diffusion of water across a selectively permeable membrane).
Worked solution
$\text{Water moves across the potato cell membranes}$
$\text{Direction of movement depends on solute concentration gradient}$
$\text{This process is osmosis}$
Answer: Osmosis (the diffusion of water across a selectively permeable membrane).

The gain or loss of mass in the potato slices resulted from water moving into or out of the cells by osmosis, depending on the surrounding solution's solute concentration.

Question 80 · 1 pt · constructed response

Explain why the potato slice in distilled water (Beaker 1) was the only one to gain mass after 30 minutes.

Model answer: Distilled water has no solutes (0% solute concentration), so it has a lower solute concentration than the inside of the potato cells (hypotonic environment). Water therefore moved into the potato cells by osmosis, causing a gain in mass. In beakers 2 and 3, the salt solutions had a higher solute concentration than the potato cells, so water moved out of the cells instead.
Worked solution
$\text{Distilled water: 0\% solute} < \text{potato cell solute concentration}$
$\text{Water moves from low solute (distilled water) to high solute (cell) by osmosis}$
$\text{Net water influx} \Rightarrow \text{mass increases}$
Answer: Distilled water has no solutes (0% solute concentration), so it has a lower solute concentration than the inside of the potato cells (hypotonic environment). Water therefore moved into the potato cells by osmosis, causing a gain in mass. In beakers 2 and 3, the salt solutions had a higher solute concentration than the potato cells, so water moved out of the cells instead.

Because distilled water contains fewer solutes than the potato cells' interior, water moved into the cells by osmosis, causing a net gain in mass, unlike the higher-concentration salt solutions which drew water out.

Question 81 · 2 pts

Base your answers on the Universal Genetic Code Chart (Messenger RNA Codons and the Amino Acids for Which They Code). Note: The answer to question 81 should be recorded on your separate answer sheet. 81 Using the Universal Genetic Code Chart, how many messenger RNA codons code for the amino acid leucine (LEU)?

live question 81 figure
Worked solution
$\text{UUA, UUG code for LEU (from UU row)}$
$\text{CUU, CUC, CUA, CUG code for LEU (from CU row)}$
$\text{Total} = 2 + 4 = 6$
Answer: 6

On the genetic code chart, leucine is coded for by UUA, UUG, CUU, CUC, CUA, and CUG, a total of six codons.

Question 82 · 2 pts

The table below shows a section of messenger RNA for five species of similar organisms. Note: The answer to question 82 should be recorded on your separate answer sheet. 82 What is the DNA sequence that codes for the messenger RNA codons shown in Species X?

live question 82 figure
Worked solution
$\text{mRNA: UUA\ CCC\ AAU\ AGA}$
$\text{Complementary base pairs: A-T, G-C, U-A(mRNA)}$
$\text{UUA} \to \text{AAT},\quad \text{CCC} \to \text{GGG}$
$\text{AAU} \to \text{TTA},\quad \text{AGA} \to \text{TCT}$
$\text{DNA sequence} = \text{AAT GGG TTA TCT}$
Answer: AAT GGG TTA TCT

Applying complementary base pairing (A-T, G-C) to each base of the mRNA codons for Species X (UUA CCC AAU AGA) gives the DNA sequence AAT GGG TTA TCT.

Question 83 · 1 pt · constructed response

Write the number of the species in the table that would produce the same sequence of amino acids as Species X.

live question 83 figure
Model answer: Species 1
Worked solution
$\text{Species X: UUA CCC AAU AGA} \to \text{Leu-Pro-Asn-Arg}$
$\text{Species 1: CUG CCC AAU AGA} \to \text{Leu-Pro-Asn-Arg}$
$\text{Both CUG and UUA code for Leucine (redundancy of the genetic code)}$
$\text{Species 1 produces the identical amino acid sequence}$
Answer: Species 1

Although Species 1's first codon (CUG) differs from Species X's (UUA), both code for leucine due to the redundancy of the genetic code, so the two species produce the same amino acid sequence: Leu-Pro-Asn-Arg.

Question 84 · 1 pt · constructed response

Base your answer on the following: A group of students designed an experiment to determine if an individual's age had any effect on pulse rate. The data collected are recorded in the table below. 84 Based on these data, the students concluded that pulse rate increases with age. State one reason why this conclusion might be questioned.

live question 84 figure
Model answer: The data do not show a consistent increasing trend, for example, the 43-year-old has a lower pulse rate (48) than several younger individuals, and the 51-year-old has the highest rate (84); also, only one individual was tested per age, so the sample size is too small to support a general conclusion.
Worked solution
$\text{Check for consistent trend across ages}$
$\text{Age 43: pulse } 48 \text{ (lower than younger ages)}$
$\text{Age 51: pulse } 84 \text{ (much higher than surrounding ages)}$
$\text{Inconsistent pattern and small sample size weaken the conclusion}$
Answer: The data do not show a consistent increasing trend, for example, the 43-year-old has a lower pulse rate (48) than several younger individuals, and the 51-year-old has the highest rate (84); also, only one individual was tested per age, so the sample size is too small to support a general conclusion.

The data are inconsistent (pulse rate does not rise steadily with age, e.g., it dips at 43 and spikes at 51) and only one subject was tested per age group, so the sample size is too small to draw a reliable general conclusion.

Question 85 · 1 pt · constructed response

Identify an organ of the human body where diffusion occurs, and identify one specific molecule that diffuses between that organ and the blood.

Model answer: The lungs; oxygen (O2) diffuses from the alveoli into the blood (and carbon dioxide diffuses from the blood into the alveoli).
Worked solution
$\text{Organ: lungs (alveoli)}$
$\text{Molecule: oxygen diffuses from alveoli into capillaries/blood}$
Answer: The lungs; oxygen (O2) diffuses from the alveoli into the blood (and carbon dioxide diffuses from the blood into the alveoli).

In the lungs, oxygen diffuses from the alveoli into the surrounding capillaries (blood) down its concentration gradient, and carbon dioxide diffuses the opposite way.

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